Kehinde Oyeniran

3 Questions 9 Answers 0 Followers

Questions related from Kehinde Oyeniran

How best to transform grasses?

I intend to inoculate some grasses I am working on with some viruses in Agrobacterium. I am considering vacuum infiltration but the problem is the grasses are quite tender and might likely not...

25 April 2019 7,941 2 View

Cutting a dimer insert?

I already dimerized an insert in a vector and now trying to cut the dimer for further cloning; since there are no suitable clean ways to this, I intend to do a full xbal digest of the vector (It...

09 May 2018 5,418 5 View

What do i need to worry about if a restriction enzyme is CpG methylation sensitive? Is there any way to make it work?

I am trying to carryout diagnostic digest with AatII and DraIII (Adei) but the two did not cut as expected. Could it be because of CpG sensitivity? The tests are the paired lanes separated by...

10 October 2017 5,036 5 View