Kecheng Zhou

4 Questions 8 Answers 0 Followers

Questions related from Kecheng Zhou

Does someone has experience with soft-agar colony-forming assay ?

Hi, I have an question regards soft-agar colony-forming assay, if firstly transit transfected miRNA mimic, then start soft-agar colony-forming assay, does that works? this looks wield for me,...

25 August 2016 4,573 3 View

How to label cells? (for separation by FACS afterwards)

Hi, i am now going to label one population(A), and doing the co-culture with another cells(B), after some treatment(about 3days), i want to separate A cells by FACS, is there somebody have...

20 June 2016 439 3 View

Do someone has experience with mTOCR1 activity?

Recently, we are testing mTOCR activity, by using 4EBP(Cell Signaling Technology #9452) and PP70 antibody(Cell Signaling Technology #9205), we have some problems. 1. there is always many...

08 May 2016 3,652 6 View

Is there somebody have experience in FRET analysis? is it normal that FRET efficiency various in different part of cell?

is there somebody have experience in FRET analysis, i recently use Leica SP8 confocal doing the FRET to detect the protein protein interaction, by using a positive Ctrl, Venus-Cerulean, we keep...

27 April 2016 4,977 7 View