KC Lee

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Questions related from KC Lee

Has anybody used the Elisa kits from Elisa Genie, LifeSpan BioSciences, Aviva Systems Biology, Abbexa and Antiboides-online?

I am looking for sandwich Elisa kits for human CTNND1, and I have found the kits from the above companies. However, our group are not familiar with these companies, and I cannot find publications...

03 February 2020 6,327 1 View

Rules for paired sample t-test with samples from cell culture?

My experiment is isolating primary cells from biopsy of different patients. And then to culture the primary cells in two conditions, one is the negative control, and the experimental set-up is...

07 July 2019 6,385 5 View

Markers for intracellular protein and surface protein. Thoughts?

Dear all, I am using this kit for isolation of cell surface protein. https://www.thermofisher.com/order/catalog/product/89881 In brief, this kit biotinylate the surface protein only, as the...

26 January 2018 2,552 2 View

What addition protection can be added to embryoid bodies suspended in PBS?

Hi All, I am trying to do biotinylation labelling on my embryoid bodies (differentiated from hESC), to label the surface proteins on it. The kit suggest diluting the biotin in PBS, and the...

04 November 2017 3,518 2 View

What restriction site should be used, between the tag and the gene of interest in cloning?

I want to design a primer to make a 2X Flag tagged protein. The forward primer would have the sequence of 2xflag and the cds of the gene. I also want to add a restriction site BglII (AGATCT)...

15 June 2015 4,024 4 View

What common false positive proteins are there in affinity purifcation and mass spectrometry?

I have recently done affinity purification and mass spectrometry. The aim is to identify new protein interactors of the protein of interest. I have the MS result from the first trial, where I...

11 April 2014 5,243 0 View

Co-immunoprecipitation of nucear proteins?

I am going to do the co-immunoprecipitation of nuclear proteins. To do extract nuclear protein in cell lysis, I need to use lysis buffer with higher ionic strength (e.g. 400mM of NaCl). Will it be...

23 April 2013 9,967 1 View