Kavita Mehta

2 Questions 2 Answers 0 Followers

Questions related from Kavita Mehta

Low RFU for amplification curve in RT-qPCR?

I am performing some RT-qPCR with RNA transcripts (HIV-integrase) and as a control (RCAS transcripts). The amplification curve for my HIV int has low y-axis values. To rule out the possibility of...

12 April 2024 8,526 1 View

How to represent qPCR data without control value?

I am checking the expression of a gene across different cell lines. I have used GAPDH as a housekeeping gene. But I cannot use the delta-delta Ct method as I don,t have a control group. How can I...

31 March 2021 3,107 3 View