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I have an Env vector (5.5kb), Gag-pol vector (12.5 kb) and a gene of interest (8.7 kb) to be transfected using lipofectamine to 293T cells. According to the protocol of lipofectamine the...
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We are facing some kind of contamination which do not have sensitivity to Pen Strep or Plasmocin. We thought it is mycoplasma as it is not seen in 4x or 10x and also stained positive for DAPI, but...
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