Kaushal Baid

3 Questions 3 Answers 0 Followers

Questions related from Kaushal Baid

Has anyone run dsRNA immunoblots?

Hi everyone I am trying to run an immunoblot for dsRNA molecules of specific lengths. I have been running 5% PAGE gels and transferring the RNA using semi-dry transfer @ less than 10V. Note: the...

23 August 2016 3,213 1 View

Ways around expressing a protein which is seemingly toxic for the cells at higher concentrations in mammalian cancer cell line?

Hi everyone Background: Expressing a membrane protein in A549 cells (lung epithelial cancer cell line) using a expression vector under the constitutive CMV promoter. I am able to detect the...

30 March 2016 1,142 3 View

How to express a fusion protein where one of the protein is a receptor protein?

Hi I am trying to express a fusion protein where one of the protein is a receptor protein and the other is a soluble protein. I have cloned them together but for some reason, I have not been able...

29 January 2016 6,954 4 View