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Questions related from Katrina Hauka
I was growing Lactobacillus spp. from different yogurts using MRS agar to determine cfu/mL in different products. I also did Gram-staining and catalase test to confirm microorganisms grown are...
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I am trying to determine the virus titre by agarose overlay assay and staining with 0.1 volume MTT (5mg/mL in PBS). The result I got was not small white dots on the blue background. The background...
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I am working with two types of viruses from the same family. I was provided with specifically designed primers to test if either of the viruses are present in the material. However, I ran a PCR...
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I ran gel electrophoresis to see if my samples have chromosomal DNA in them (for later PCR usage). When I saw the results I didn't know how to interpret them. I understand the first line is the...
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I have infected A549 cells with two different types of Adenovirus. Today is the 3rd day post-infection. I would like to isolate viral DNA and also do viral titration but only later, someday next...
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By accident I left my cell material after using TRIzol at room temperature for about 20 hours. When I realized it was left outside, I immediately put it at -70 C. However, I think my cell material...
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Dear researchers, We are currently trying to make plant extracts using ultrasound in water. It includes many filtrations though different size filters. The smallest one used is 7-8 um pore size....
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