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Questions related from Katrina Hauka
I was growing Lactobacillus spp. from different yogurts using MRS agar to determine cfu/mL in different products. I also did Gram-staining and catalase test to confirm microorganisms grown are...
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I am trying to determine the virus titre by agarose overlay assay and staining with 0.1 volume MTT (5mg/mL in PBS). The result I got was not small white dots on the blue background. The background...
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I am working with two types of viruses from the same family. I was provided with specifically designed primers to test if either of the viruses are present in the material. However, I ran a PCR...
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I ran gel electrophoresis to see if my samples have chromosomal DNA in them (for later PCR usage). When I saw the results I didn't know how to interpret them. I understand the first line is the...
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I have infected A549 cells with two different types of Adenovirus. Today is the 3rd day post-infection. I would like to isolate viral DNA and also do viral titration but only later, someday next...
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By accident I left my cell material after using TRIzol at room temperature for about 20 hours. When I realized it was left outside, I immediately put it at -70 C. However, I think my cell material...
07 February 2013 253 15 View
Dear researchers, We are currently trying to make plant extracts using ultrasound in water. It includes many filtrations though different size filters. The smallest one used is 7-8 um pore size....
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