Katie Boucher

2 Questions 3 Answers 0 Followers

Questions related from Katie Boucher

PGEX-6p-1 cloning: insert gene by restriction digestion and T4 ligation, can I transform the ligation in DH10B or stellar cells?

I know pGEX plasmids are best expressed in BL21 cells for protien expression, and the culture is induced with IPTG, but when I"m initially cloning the plasmid, does it make a difference to...

09 September 2019 1,038 4 View

What are your suggestions to close up a vector after removing a fragment by restriction digest?

Dear community, I am working with a HUGE protien, in a plasmid we received from collaborators. It is 20,880 bp in size, and the gene of interest is aprox. 15,600 bp. Thankfully the received...

08 August 2018 3,498 5 View