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I have a pDonr221 vector, that when sequenced clearly has the ccdB (Cell death gene) - so normally would only be able to propigated in cell death resistant E.coli strains. Recently, when I do a BP...
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Dear community, I am working with a HUGE protien, in a plasmid we received from collaborators. It is 20,880 bp in size, and the gene of interest is aprox. 15,600 bp. Thankfully the received...
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