How can I transfer free ubiquitin as well as poly-ubiquitinated proteins to the same membrane?

Hi, I would like to visualize both poly-ubiquitinated protein species (from 63 kDa to 200 kDa) as well as free ubiquitin (8.5 kDa) on the same Western Blot. I’d be using a 4–15% Mini-PROTEAN® TGX™...

01 December 2015 1,388 3 View