How long should be the 3'UTR region to perform a luciferase assay?

The vector I'm using is pmirGLO (7350pb) and my 3'UTR region has 2300pb (yes, it is very long). I am testing the interaction of 2 miRNAs, both have two binding sites to the 3'UTR region, two of...

04 April 2018 8,492 2 View