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I have mouse lung tissue embedded in paraffin blocks, and I am trying to slice them to make slides. I did not fix these tissues, so I cannot speak to the fixation process at all, or anything that...
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I will be using ultrathin sections of mouse lung tissue as a sample for TEM imaging. I have no experience with TEM techniques, but we are looking for the best fixation solution to use...
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I am trying to stain paraffin-embedded tissue slides using primary + secondary antibodies, and also a phalloidin actin probe. My plan is to deparaffinize the tissue slides (in this case, mouse...
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I need to prepare a 0.2 M phosphate buffer. In my research, I've seen people prepare it with both monobasic and dibasic sodium phosphate... is this necessary? Can I just use one or the other?
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