After plating mouse primary neonatal cardiomyocyte we do not observe beating after a few days, any suggestions as to how we can trouble shoot?

We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...

21 June 2024 351 0 View