Kate Gates

3 Questions 19 Answers 0 Followers

Questions related from Kate Gates

Why do I have so little events compared to the number of cells I plate?

I plate around 800,000 cells (PBMCs) in a well in a 96well plate, but when I read them on the LSR-II about 4 days later I only get 250,000-150,000 events total, half of which is viable, only 10%...

05 May 2015 6,228 13 View

Why are my lymphocytes dying in culture post RBC lysis?

Hello, I am isolating lymphocytes from murine peripheral blood with ACK RBC lysis buffer following manufacturers instructions, labeling them with CellTrace Violet (CTV), and then culturing with or...

04 April 2015 5,127 25 View

Why are my unstimulated cells still proliferating like my stimulated ones?

I am culturing murine PBMCs with CFSE for 4 days along with ConA (1ug/20uL) and I have an unstimulated control but when I read my cells on flow the CFSE signal from my unstimulated cells is around...

01 January 2015 4,795 16 View