Katarzyna Dziedzic

2 Questions 12 Answers 0 Followers

Questions related from Katarzyna Dziedzic

What is proper gating strategy for LIVEDEAD stained population in order to exclude dead cells from analysis?

Hi! I would like to discriminate dead cells from my population using LIVEDEAD fixable dye. In principle it stains amine residues in both live and dead cells, but because of cell membrane...

10 October 2017 9,590 4 View

Has anyone tried freezing cell pellets in TRIzol?

I'm performing combined RNA isolation protocol: phase separation by TRIzol and chlorophorm and spin (silica) collumn one from adherent tissues. I was wondering if I can store cell pellets...

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