Kassondra Hickey

3 Questions 6 Answers 0 Followers

Questions related from Kassondra Hickey

Why choose hexadecane/span80 over light mineral oil/span80 for microfluidic emulsion?

Hi guys, I am curious as to why someone would choose certain combinations of oil phase and surfactant over others. For example, I am currently using light mineral with span 80 added in my...

04 April 2019 763 4 View

Why are my primary mixed glia not proliferating?

I have isolated primary mixed glia from P0-P2 mouse cortices and use trypsin for dissociation. I am trying to expand my cultures for qPCR experiments. So far I have not had luck with my cell...

03 March 2018 7,757 7 View

Why does my old antibody produce nuclear fluorescence when it should be cytoplasmic?

I am doing ICC on some cultured astrocytes with an A2B5 antibody. From my understanding, this should be expressed on the cell surface and throughout the body. I have performed the stain multiple...

07 July 2017 8,331 2 View