Point Mutation Question: How should I go about designing primers for a target sequence with extremely high GC% ?

I have a pLEX 307 plasmid that has 3 BsmBI sites that I am trying to remove. 1 site is in the SV40 promoter, another site is in the PuroR sequence, and the last in the CmR sequence. I was able to...

25 April 2025 4,252 1 View