Kashif Shamim

5 Questions 12 Answers 0 Followers

Questions related from Kashif Shamim

Can iodoacetate enhance the protease activity instead of inhibiting?

While checking the effect of inhibitors on protease enzyme it was found that iodoacetate instead of inhibiting the protease activity it has enhanced the protease activity. I searched alot but...

13 March 2018 6,593 3 View

I need help in finding out the best Taq DNA pol. that's suitable for amplifying 4 kb fragment of gene of interest. Please mention the company also?

I would like to amplify 4 kb fragment of gene of interest. In order to do so I am little bit confused as to whether I should go for normal Taq DNA pol or some thing else. My point is, will normal...

04 January 2016 7,420 5 View

Can you help me out with expression vector pET32a?

I am trying to clone a gene in expression vector pET 32a but while doing so when I run a gel (0.8 agarose) with cut and uncut vector I got some weird result. The cut vector resulted in band of 4...

28 May 2015 277 10 View

How to clone a gene for restriction enzyme?

I would like to clone a gene which codes for restriction enzyme Sau 3AI but I am not getting the primer sequence. Can anyone help?

15 December 2012 8,638 4 View

What is the best procedure used for ligation?

I read quite a few procedures for the ligation process but I am confused which one is the best or which procedure gives the best ligated result?

19 October 2012 1,882 8 View