Kashfia Ahamed

2 Questions 3 Answers 0 Followers

Questions related from Kashfia Ahamed

Measuring Kd value from absorbance curve of protein with its chromophore?

I am working with this protein which gives fluorescence after bonding with its chromophore. I have measured the absorbance change after addition of protein to the chromophore to follow the...

15 November 2018 1,896 3 View

To find the concentration and weight of a purified protein from UV absorption results?

I purified smURFP was concentrated to be ~3.5 mL. and measured absorption spectrum of the sample by diluting 100-times in a buffer. Absorbance at 280 nm of the diluted sample was 0.132 for...

30 August 2018 4,339 3 View