After protein percipitation from acetone, can I sonicate to resolubize it for lc-ms?

Hi, I am trying to find effecient way to resolubize the protein. We currenetly use 8M Urea with 50mM DTT and incubate at 37 for 30 min. I do hand pipet and vortex 15 sec before and once in the...

20 September 2023 8,260 0 View