Karim Abdelkader

3 Questions 10 Answers 0 Followers

Questions related from Karim Abdelkader

What is the best universal buffer system to compare enzymatic activity at different pH points?

Hi everybody, I am planning to test effect of pH on endolysin (phage enzyme) activity, so I have to prepare solutions with different pHs. To delete effect of buffer components on enzymatic...

13 September 2018 585 10 View

Phage precipitation using PEG and NaCl

I have precipitated phage as pellets using PEG and NaCl method then i have suspended the pellets using 5ml SM buffer with gelatin, lytic activity of the phage was confirmed using spot test, after...

15 August 2017 9,652 2 View

How to store bacteriophage for long time??

After screening for plaque formation, plaques are formed,then I have touched the plaque with sterile Pasteur end and dispersed in in 200ul of PBS and stored it in refrigerator. After repeating...

08 July 2017 1,535 12 View