Karen Mention

3 Questions 3 Answers 0 Followers

Questions related from Karen Mention

What could be the reasons for a reverse insert in Gibson Assembly?

Hello, My labmates designed primers for a Gibson assembly experiment. They did the way they usually do, making a final plasmid and using benchling to design the primers. As a result of the...

13 December 2024 9,926 2 View

Escherichia strain suddenly becoming resistant to 2 antibiotics, how can it be possible?

Hi, My lab was doing experiment with a Escherichia strain. It seems it was working well, but after about just 1 month, the negative controls for transformation became positive to Kanamycin and...

28 August 2024 7,317 4 View

Does Spectinomycin lose its efficacy if the aliquots are stored at -70°C ?

A student made Spectinomycin stocks (60mg/ml) and stored them at -70°C for about a month. Would that make the Spectinomycin inefficient? Thank you

26 August 2024 5,305 0 View