Kara Hood

3 Questions 2 Answers 0 Followers

Questions related from Kara Hood

What are similar detergents to Na-deoxycholate and Lauryl sarcosinate I can use to solubilize a protein for IMAC?

In screening detergents that solubilize my protein of interest from IM preps in E. coli (after overexpression from a pET vector), I found that Na-deoxycholate and lauryl sarcosinate are the most...

02 December 2020 5,931 6 View

Can I use negative-stain TEM to screen liposome integrity?

My question is focused on systematically troubleshooting liposome composition and detergent for cryoET analysis. I attached the paper by Yao et al 2020 (PNAS) where they began this process, but...

30 September 2020 8,457 2 View

I accidentally let a bacterial transformation outgrow overnight. Can I have any hope for successful transformations, or should I just redo it?

I transformed with a ligation reaction into Ecoli comp cells, and let them outgrow in a couple mLs of LB overnight on accident. Can I plate the transformations anyway and hope that I have colonies...

19 April 2018 847 4 View