Kara Hood

2 Questions 1 Answers 0 Followers

Questions related from Kara Hood

What are similar detergents to Na-deoxycholate and Lauryl sarcosinate I can use to solubilize a protein for IMAC?

In screening detergents that solubilize my protein of interest from IM preps in E. coli (after overexpression from a pET vector), I found that Na-deoxycholate and lauryl sarcosinate are the most...

02 December 2020 5,891 6 View

I accidentally let a bacterial transformation outgrow overnight. Can I have any hope for successful transformations, or should I just redo it?

I transformed with a ligation reaction into Ecoli comp cells, and let them outgrow in a couple mLs of LB overnight on accident. Can I plate the transformations anyway and hope that I have colonies...

19 April 2018 833 4 View