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Questions related from Kanti K Yadav
I have already prepared znO Nanoparticles. Can anyone suggest me a method to coat them with chitosan without changing its size morphology. thanks
12 December 2016 9,217 5 View
Hi, My protein is unstable and form aggregtes after purification (even with 300mM salt, 0.5mM TCEP and 5% glycerol). Protparam data gives an instability index of 42 stating it unstable. However I...
03 March 2016 3,250 11 View
Hello everyone, I have been trying to express and purify a protein. I am getting a good protein yield after Ni-NTA chromatography, as seen in SDS-PAGE. Problem is after purification, during...
08 August 2015 1,159 7 View
Hello, I have to perform dialysis after Ni-NTA purification. Can I reuse a dialysis membrane. It has been kept in a sodium azide solution(1%) fir past six months at 4 degree. If yes, what is the...
07 July 2015 9,355 8 View
I am working with a protein which is told to be secretory and has been purified under native conditions. But When I expressed and induced, it formed inclusion bodies( white preicipitate after...
05 May 2015 1,366 7 View
hi, I am trying to express and purify a His-tagged protein of interest. I am using pET vector and BL21DE3 strain for expression. At first, I was able to express the protein with .5mM IPTG for 5h...
04 April 2015 9,136 4 View