Kanti K Yadav

6 Questions 23 Answers 0 Followers

Questions related from Kanti K Yadav

Method for nanoparticle coating with chitosan?

I have already prepared znO Nanoparticles. Can anyone suggest me a method to coat them with chitosan without changing its size morphology. thanks

12 December 2016 9,204 5 View

What are the chances of a natively unstable protein to refold effeciently after purification under denaturing conditions?

Hi, My protein is unstable and form aggregtes after purification (even with 300mM salt, 0.5mM TCEP and 5% glycerol). Protparam data gives an instability index of 42 stating it unstable. However I...

03 March 2016 3,230 11 View

How can I troubleshoot protein precipitation after purification?

Hello everyone, I have been trying to express and purify a protein. I am getting a good protein yield after Ni-NTA chromatography, as seen in SDS-PAGE. Problem is after purification, during...

08 August 2015 1,145 7 View

Can dialysis membranes be reused?

Hello, I have to perform dialysis after Ni-NTA purification. Can I reuse a dialysis membrane. It has been kept in a sodium azide solution(1%) fir past six months at 4 degree. If yes, what is the...

07 July 2015 9,341 8 View

Is it possible for a secretory protein to form inclusion bodies, during induction? if yes, then under what conditions?

I am working with a protein which is told to be secretory and has been purified under native conditions. But When I expressed and induced, it formed inclusion bodies( white preicipitate after...

05 May 2015 1,351 7 View

Can anybody help me with purification of a his tagged protein?

hi, I am trying to express and purify a His-tagged protein of interest. I am using pET vector and BL21DE3 strain for expression. At first, I was able to express the protein with .5mM IPTG for 5h...

04 April 2015 9,122 4 View