Kanomi Sasaki

2 Questions 4 Answers 0 Followers

Questions related from Kanomi Sasaki

If a protein is easily aggregated during dialysis in HBS, does that infer that the protein would easily crystallize?

I am working with a protein and had to dialyze the solution to remove salt. Even though, during purification and AcTEV digestion, the proten did not aggregate, during dialysis, it obviously...

10 October 2016 3,604 7 View

Why are there no bands in my IP lanes?

I performed an IP to pull-down an endogenous protein A. When I immunoblotted with anti-protein A, the film only showed bands for the input lanes. The first chemiluminescence treatment was with...

08 August 2016 9,472 7 View