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Questions related from Kanomi Sasaki-Capela
Hello, I am trying to isolate individual colonies using Fisher paper cloning discs. I soak the discs in Trypsin, place on top of colony, and the cells easily attach to the paper. When I transfer...
28 November 2018 3,702 0 View
After PCR using Q5 pol, I ligate the insert into pCR-Blunt using T4 ligase. Transformation into DH10 is successful and a few colonies are mini-prepped. The plasmid is then cut with EcoR1-HF and...
11 January 2018 2,601 8 View
