Kanokwan Sakolpongpairoj

2 Questions 2 Answers 0 Followers

Questions related from Kanokwan Sakolpongpairoj

What is the proper population of cells for calculate population doubling time?

If fibroblast cells had been washed and flushed the floating cells out before dissociation and staining process, should only viable cells or total cells be counted for PDT calculation ?

01 January 2015 4,077 2 View

Can low 260/230 ratio in RNA extraction interfere with qPCR or RT-PCR result?

I extracted human embryoid body RNA for PCR analysis using Trizol reagent. RNA conc. is between 50-200 ng/ul, and 260/280 ratio is about 1.7-2.1,so these are really good, but 260/230 ratio is...

09 September 2014 4,258 6 View