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I am back with my problem on site directed mutagenesis using stratagene quickchange protocol. I really need some more help. The following is my situation : My target gene is ~1.1kb and it is in...
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I have been trying to digest a vector carrying an mCherry tag using SgrBI (as an alternative to SacII), but I am failing in my attempts. The reaction that I set was as follows: 6ul SgrbI buffer...
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Is ir safe to preserve a ligation reaction at -20°C (without heat inactivating or anything) before doing my transformation?
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Despite having designed quite a number of primers in the past, I am still not completely clear of the idea when it comes to the designing of a reverse primer! where exactly should I add my...
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