Kannan M.

3 Questions 9 Answers 0 Followers

Questions related from Kannan M.

Is environment PH simulation the better one or is protonated protein PKa simulation better for acid protein?

I have a acidic protein that is proven to be stable and functional in acidic environment. But I am confused on whether I have to carry out the simulation using environment PH or by protonating the...

13 January 2015 6,525 2 View

Problem in Membrane protein simulation

I have been running a membrane protein simulation. I followed the tutorial on: http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin/gmx-tutorials/membrane_protein/index.html But I faced a...

26 January 2013 9,199 7 View

How can I overexpress the target protein using mammalian expression vector?

I have plasmid of my target protein which is a mammalian expression vector, PCDNA3 with Ampicillin marker. I want mass number of target protein for crystallization work. Does anyone have any...

29 October 2012 1,105 9 View