Kang Yi Lee

3 Questions 6 Answers 0 Followers

Questions related from Kang Yi Lee

High background for Opal uniplex staining?

Hi, I am currently optimizing the staining for CD137 on mouse HCC/spleen (also known as 4-1BB) using Opal immunofluorescence system. My blocking steps include (1) 5% non-fat milk for 30 mins (2)...

26 November 2021 5,445 1 View

How can I remove cell surface-bound RNA?

Hi, I am currently treating T cells with CD137-targeting aptamer to examine if the aptamers can specifically bind to CD137 receptor to be internalized. I performed my experiment by treating my...

05 July 2021 2,269 4 View

During LDHA purification using Ni2+-NTA chromatography, impurities were still found in protein, how can i improve?

For LDHA enzyme purification using Ni2+-NTA chromatography, the following buffers were used: (1) Ni binding buffer with 20mM imidazole (2)Ni washing buffer with 100mM imidazole. However,...

30 October 2017 7,830 7 View