Kaneez Fatima

2 Questions 2 Answers 0 Followers

Questions related from Kaneez Fatima

Is mechanical method (gentle pipetting) for detaching HEK293T cell is fine? Or it damages the cells?

I have to count live and dead cells after drug treatment. So I was wondering if resuspension (detachment) of cells with pipetting will affect cells or it should be first washed with PBS and then...

19 August 2021 3,117 3 View

What is the best and more successful way for making knockout cell line by using CRISPR Cas9 method?

I am working on HEK293T cells and trying to make gene specific knockout cell line by using Crispr Cas9, but at the end of single clones the success rate is very low.

28 July 2021 7,460 3 View