Why do I get higher band on CBB staining after cleaving my GST tagged protein with precision cleavage?

I have cloned a gene in pGEX6p2 with N-terminal GST, C-terminal Flag tag and expressed in E.Coli BL21 with 0.1 mM IPTG, 30degree celcius at OD600-0.5, induced for 3 hours. After GST tag...

08 December 2019 6,773 0 View