Kamlesh Kumar Soni

4 Questions 3 Answers 0 Followers

Questions related from Kamlesh Kumar Soni

Can anyone share the paper entitled "Quantitative GUS Activity Assay in Intact Plant Tissue" ???

hi I am looking for this paper.. Can anyone share this paper pls.?

03 March 2018 4,500 0 View

How to check stability of protein(Transcription factor)??

Hi I have been trying to purify Transcription factor. It is N terminal His tagged protein expressed with pET28a in E.coli. strain BL21(DE3) codon plus. I am looking for best and easiest way to...

04 April 2017 5,840 0 View

Why does protein degradation occur during isolation and purification? Find attachment?

Hi! I have been trying to purifiy plant transcription factor using GST tagged in strain BL21(DE3) codon+. But protein is coming with two sharp band in supernatant and elutions also.In elution some...

02 February 2016 4,966 3 View

I have a transcription factor which I need to purify but it is going to the insoluble fraction?

I tried pEt 28 and pET 32 with different temperatures and IPTG concentrations.

10 October 2014 2,524 4 View