Does anyone have PBMC nucleofection/transfection tips?

I'm trying to transfect primary PBMCs with siRNA in a Lonza 4-D Nucleofector, but I've had no luck. So far, I've been using Lonza's Amaxa unstimulated T-cell protocols, in the 20 uL/well 2x8-well...

08 August 2023 3,699 0 View