Kamelia Mirdamadi

6 Questions 4 Answers 0 Followers

Questions related from Kamelia Mirdamadi

Any suggestions on the effect of 1mM sodium citrate solution on reverse transcritpion?

I started using "RNA storage solution (1mM sodium citrate) from THermoFisher to dissolve RNA pellets and store in -20 or -80. However, there is no amplification in the Q-PCR. it is frustrating and...

11 November 2019 5,150 3 View

RNA Quality and formaldahyde Agarose gel?

I am trying to run RNA on 1% agarose/formaldehyde gel using 5x MOP buffer and SybrSafe staining instead of ethidium bromide. what is the proper voltage and time? it says 5-6V/cm, but how can I...

10 October 2019 8,391 6 View

Protein extracts not dissovled in buffer?

Hi, My samples (placental tissue) were collected and stored using RNALater. in the process of protein extraction and at the end, I was not able to dissolve the protein pallets in the homogenizing...

07 July 2019 5,155 5 View

Concentration calculation in multi-analyte ELISA?

Hello, I did multi-analyte ELISA to detect the concentrations of several cytokines, but how should I calculate the concentration as each well represents a different sample and a different...

07 July 2019 5,061 2 View

How long is the 1:1 PCR primer mix valid for?

Does primer mix expire?

06 June 2018 2,895 0 View

RNA Quality and formaldahyde Agarose gel?

I am trying to run RNA on 1% agarose/formaldehyde gel using 5x MOP buffer and SybrSafe staining instead of ethidium bromide. what is the proper voltage and time? it says 5-6V/cm, but how can I...

01 January 1970 5,984 3 View