Kamaluddeen Garba

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Questions related from Kamaluddeen Garba

Low protein yield with LAD2 cells?

I am doing an SDS PAGE with LAD2 cells lysate but the total protein obtained was 0.4ug/ul when 200ul of RIPA was used to lysed 5 million cells. Sample was lysed for 45 minutes on ice. Kindly help.

02 February 2020 6,614 3 View

Methods for concentrations to be used in immunohistochemical studies for a test compound?

I need a protocol to determine an optimum concentration to use in cell culture studies.

02 February 2018 962 0 View