Kamalika Roy Choudhury

2 Questions 5 Answers 0 Followers

Questions related from Kamalika Roy Choudhury

How do I amplify of full length HCV NS5B?

Hi all, I am struggling to amplify full length non-structural NS5B region (1.8kb) of HCV virus. I am using platinum super-fi taq polymerase to amplify this region. Also modify the cDNA synthesis...

21 April 2021 3,189 3 View

How do I perform 2D SDS-PAGE with immunoprecipitated samples?

I'm running 2D SDS-PAGE after immunoprecipitation. I'm eluting the protein complex with IPG strip rehydration buffer. However I feel I'm not able to complelely elute the complex from bead. Any...

20 April 2015 8,089 6 View