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I want to perform co localization study of protein of interest with flag Tricellulin protein. i am currently using flag anti mouse antibody which is not working for immunostaining. can any one...
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Hi i am going to do co-ip 1st time. i need its optimized protocol. i also want to ask for co.ip ,will i co transfect my cells with bait and prey protein or separate transfection?
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