Kalpita R Karan

16 Questions 103 Answers 0 Followers

Questions related from Kalpita R Karan

Are there tools to compile vcf filles containing multiple mutations/person and finally get a merged file?

I am able to obtain multiple vcf files detecting mutations in individuals. Each person has similar chromosomal but different mutation. I want to merge all these output files to either a csv or...

22 March 2020 1,766 6 View

Which cells types respond to LPS-stimulation in whole blood?

If one does not purify cells from whole blood but stimulates whole blood, which cell types are stimulated by LPS ex vivo? Apart from just looking at the stimulatory effects of LPS, I am trying to...

21 April 2019 2,450 6 View

Does DNase act on mitochondrial DNA when they are cytoplasmic?

Are there differences in nuclear and mitochondrial DNA digestion by DNases? I want to test if enzymes can digest mtDNA. If they do, then is it with similar propensity to nuclear or there may be...

09 December 2017 3,925 4 View

What are the receptors in our scalp that can sense temperature, either dry heat or heat due to pouring of water? Are they same as skin receptors?

How does brain sense teh temperature through scalp? Any ruote map? From skin, we have fibres running from skin through spinal cord and to brain reaching somatosensory cortex. Is it same when...

05 December 2014 9,627 3 View

Can NMDA currents be blocked by some ways while performing whole cell patch clamp recordings in mammalian cells?

Can removal of Mg2+ ion from external buffers help in this regard? How much endogenous glutamate influences NMDA activation. My holding potential is -80mV.

05 November 2014 3,945 12 View

Is it a good idea to study expression of protein in Immunofluorescence where one is in a fusion GFP form and the other at endogenous levels ?

I am trying to see colocalization of two proteins in confocal where one is a fusion GFP transfected and the other stained for the endogenous and detected using Alexa 568. I am no able to see...

06 June 2014 5,031 3 View

Is anyone working with electrophysiology data of glutamate transporters?

I am looking for some inputs on pulsing protocols for glutamate induced anion currents in whole cell patch clamp.

10 May 2014 7,989 11 View

Can saturation kinetics experiment and radioligand uptake experiment give a different result?

At a particular conc. I am getting different values of uptake rate in two different experiments. One is uptake experiment where 'X' is used in 10uM conc. Another is saturation kinetics from 5-50uM...

19 September 2013 7,481 6 View

Can anyone suggest the best endogenous marker or control for plasma membrane fraction in a western blot? Can it be used as a membrane marker in IF?

I am looking for some marker suggestions for using it in confocal as well as western blotting enriching the membrane. I work with rat glioma cell line as well as HEK293T cell line.

06 August 2013 8,798 10 View

What is the best solvent for Carbamazepine (AED) - DMSO or ethanol?

I want to use AED CBZ for my experiments and treat cells with this drug. I want to know what is the best solvent for it. I face some problems determining the right conc. of the drug.

13 March 2013 4,776 1 View

Can a fluorescent antibody detect a native protein in live cells?

If the cells are neither fixed nor permeabilized, can a protein specific antibody bind and can an Alexa based fluorophore recognize it? Can live cell imaging work without a GFP-fusion protein or...

06 November 2012 4,882 5 View

Has anyone got experience in isolating protein from TRIzol step?

While I am following the trizol method for protein isolation during RNA isolation from EBV cell lines. I am curious as to what is the best method to obtain proteins simultaneously while processing...

23 October 2012 4,066 14 View

Does anyone use the automated patch clamp, the Port-a-patch?

Any experience in capturing Na+ currents in HEK293 or SHSY-5Y cell lines where the current is induced by ligand bonding and transport? Na+ acting as a symporter to the membrane protein which helps...

21 October 2012 6,832 10 View

Is there any way to understand the neurotransmitter uptake ability through receptors using imaging or biochemical assays in mammalian cell lines?

Can temperature (especially high temperature) play a role in this phenomena of neurotransmitter binding, internalization or receptor dynamics?

13 October 2012 6,426 9 View

Whole cell patch clamp in mammalian cell lines eg. HEK 293, to capture Na+ currents. What is the feasible protocol?

What are the important points to be noted while looking at the uncoupled anion currents? The assay will require transfection and over-expression of a membrane protein. A ligand induced current...

11 October 2012 3,448 18 View

What is the best way to visualize membrane proteins without permeabilizing cells like COS 7 or HEK 293?

The basic idea being detection using two colors and see colocalization on the membrane. How to see membrane part first without permeabilizing and then intra-cytoplasmic part too with triton may be...

20 September 2012 4,408 9 View