Kallon Crowther

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Questions related from Kallon Crowther

Optimizing BDA Tracer Staining w/ streptavidin?

I'm using biotinylated dextran amine (BDA) as an anterograde tracer in rats that have SCIs. I injected it into the primary motor cortex (2 injection sites, 1 ul each) and I'm having trouble...

11 April 2025 1,572 0 View

How many cells do I REALLY need for intracellular staining for flow cytometry?

I'm planning an experiment where I can access intracellular cytokines in a specific subregion of the brain in mice. However, this brain region is quite small, maybe 50,000 cells per animal. I know...

21 March 2024 7,350 3 View