Kalkal Anil Trivedi

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Questions related from Kalkal Anil Trivedi

How to prevent cell clumping in matrigel invasion assay?

I am working with a cell line that migrates very well in transwell migration assays, but when I try to do an invasion assay, the cells clump together within 2-3 hours after plating. I plate them...

05 May 2016 2,463 2 View

What is the best way to isolate cancer stem cells from cancer cell lines?

Best way to identify cancer stem cells from in vitro cancer cell lines?

06 June 2014 6,276 1 View

Have there been any reports of interaction of caffeine and adenosine other than on adenosine receptors?

Caffeine and Adenosine interactions other than at Adenosine receptors with theparticular effect on intracellular signalling.

10 October 2013 472 1 View

Dark bands on edges of Western Blot and faint development of bands in the center of WB, even in the loading control?

I am trying to replicate an experiment previously conducted, and observed that irrespective of the samples loaded, on developing the WB by chemiluminescent substrate, the bands on both edges of...

09 September 2013 8,481 44 View

I work with a membrane transporter which cannot be heated before running a western blot. What can be used as an appropriate loading control?

Common loading controls like Tubulin or GAPDH need to be heated at 100 degree Celsius before loading them onto the SDS-PAGE gel. This causes the problem of me not being able to use the same sample...

09 September 2013 9,680 4 View