Kalaivani Manibarathi

4 Questions 4 Answers 0 Followers

Questions related from Kalaivani Manibarathi

Roche Lightcycler 480 error?

I get an error during relative quantification analysis in Lightcycler 480 software: Error calculating subordinate analysis, index exceeds matrix dimensions. Has anyone encountered and solved this...

11 December 2022 5,979 0 View

Reprogramming fibroblasts with episomal vectors_how often is Vector integration possible?

I am reprogramming human dermal fibroblasts to iPSCs using Yamanaka factors with Nucleofection method. All obtained iPSC clones shows integration of one or a few plasmids after passage 6 of the...

15 August 2021 1,154 3 View

Is there a good online tool available to check the strength of Kozak consensus sequence for translation initiaiton, given the amino acid sequence?

I have an amino acid sequence of my target (KO) and there are different potential ORFs that could be initiated, as predicted by expasy-translate. Is there a way to check the strength of the Kozak...

04 May 2021 7,339 5 View

How to detach iPSC colonies from reprogrammed fibroblasts?

I am reprogramming fibroblasts to iPSCs. I see small iPSC colonies around D21 and want to isolate them. I have two questions here. 1. Many iPSC colonies are already yellow from the center. Does...

02 March 2021 9,913 5 View