Kakali Ghoshal

3 Questions 4 Answers 0 Followers

Questions related from Kakali Ghoshal

Hepatocyte harvest issues with High fat Diet mouse?

Hi, I am trying to harvest hepatocytes from high fat fed C57BL/6 mice. Because of some genetic manipulation and high fat diet, they are actually pretty obese. I find it difficult to make the IV...

15 March 2023 8,102 3 View

How to increase binding for a very weak antibody?

I am working with total Foxo1 protein and I am using an antibody from Cell Signaling (Foxo1 (L27) Antibody #9454)). However, the antibody seems to give very very faint band around 80 kD for liver...

27 May 2020 9,514 12 View

Why I am getting very low viable mouse primary hepatocytes?

Hi, I am isolating primary mouse hepatocyte. The problem I am facing is sometimes I am getting very low viable cells (80%). The problem is particularly occuring with one specific strain so this...

07 December 2019 2,193 3 View