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Questions related from Kaiyuan Ni
I am trying to breed Rag-/- mice and I found high percentage of female dead by dystocia. Is there any way to avoid this issue?
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I've tried several times to do western blot of HIF1-alpha but failed. Has anyone managed to get good bands of HIF1-alpha under hypoxic condition using Abcam antibody (1:500) in a glove box? Can...
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Rag2-/- mice are more immunodeficient than nude mice, though both show highly activated NK. However, few literature reported human-derived tumor bearing Rag2-/- for cancer diagnostics and...
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I'm doing some in vitro studies that need to culture cells with low PBS concentration to mimic plasma. Is there any commercial available medium with lower PBS concentration (1/5 than normal)? Thanks.
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