Kaushik Thakkar

2 Questions 6 Answers 0 Followers

Questions related from Kaushik Thakkar

Enzyme/Protein stability - at -80 degrees

Hello Everybody, I am working on a enzyme and its substrate. The enzyme is in the form of a membrane preparation (Sf9 ER fraction) and is resuspended in Hepes-NaOH (pH = 7.0) after...

01 January 2010 9,055 3 View

Protocol for Histag based Ni-NTA protein purification

Hello everybody, I need your suggestions for the protocol that i have used before. My protein size is ~12 kDA, it is N-term GST, C-term His tagged. I planned to use Ni-NTA. As you know,...

01 January 2010 6,228 3 View