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Questions related from Jyoti Verma
Hey I am currently trying to clone a gene of 1000bp into pet28a by using the BamH1 and XHOI restriction sites. These sites are introduced into my gene by overhang PCR. Restriction are done....
29 April 2025 5,456 3 View
I am trying to label RNA with 4SU (by IVT) and then doing Poly A tailing. However, i can see 4SU labelling of my RNA in IVT step but when I do Poly A tailing. I find it difficult to isolate the...
12 March 2025 9,126 0 View
Hey, I am isolating RNA from the IVT product. Earlier I used to extract by adding simply chloroform and precipitation by Isopropanol. However, the yield comes in ug but in gel, the RNA is 1/10 of...
10 March 2025 5,273 3 View
