Jyoti Sawant

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Questions related from Jyoti Sawant

How can I troubleshoot the lack of surface expression of CAR in HEK293T cells after lentiviral transduction?

Details: I have developed a Chimeric Antigen Receptor (CAR) construct with an HA tag for detection, and it was cloned into a lentiviral plasmid containing ZsGreen and luciferase reporter genes. I...

07 January 2025 4,905 2 View

How can I effectively transduce HEK293T cells with lentivirus?

I am working on transducing HEK293T cells with a lentivirus that I produced to express a specific gene of interest. I would like to know the best practices for achieving efficient...

07 January 2025 3,734 2 View

How can I conjugate DNP to an IgG antibody in a 1:5 molar ratio?

I want to conjugate DNP (2,4-dinitrophenyl, molecular weight: 394 Da) to an IgG antibody (molecular weight: 150 kDa) in a 1:5 molar ratio (1 antibody to 5 DNP molecules). I would like to know: How...

07 January 2025 6,318 0 View