Does 2M Arginine/2M Proline in protein refolding buffer (pH 8.0) will interfere with enzymatic activity

I am refolding a hydrophobic protein, a fusion tag (size: ~12 kDa; PI: 5.8) from solubilized IB in 8M Urea. The refolding buffer consists of 100mM Tris-HCl, 100mM EDTA, 2M Arginine, 2M Proline, 2%...

02 August 2017 6,418 2 View