J.W.Nirmani Yasara

2 Questions 2 Answers 0 Followers

Questions related from J.W.Nirmani Yasara

Does fixing stem cells in formaldehyde before conducting flowcytometry anaslysis affect the outcome?

Hi, I'm planning to quantify cells with different receptor expressions in stem cells throughout the 21 cell culture phase. Can I fix cells in 4% formaldehyde on selected days and conduct the flow...

06 August 2021 7,056 3 View

Can I use cells stored in RNAlater directly for RNA isolation?

I'm planning to isolate RNA from cells stored in RNAlater with Qiagen mini kit. In one article, I saw we can directly use cells in RNAlater without washing it with PBS (but needs to put a higher...

25 May 2021 919 3 View