Justine Calise

4 Questions 9 Answers 0 Followers

Questions related from Justine Calise

Why can I not digest or sequence my plasmid?

I amplified a plasmid and now I have an eluted stock from a maxi prep i did. I sent the plasmid out for sequencing and it came back as no priming. I tried a restriction digestion with 2 different...

14 August 2015 4,242 6 View

Any suggestion on the seeding density for primary monocytes in a 96-well?

I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...

04 June 2015 6,501 2 View

How do I stimulate cell cultures with beta-estradiol?

I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...

16 May 2015 3,024 6 View

Can I use DataAssist or ExpressionSuite software for analysis if I acquired my qPCR data on the LightCycler 480?

As opposed to using Excel for analysis.

24 January 2015 5,275 1 View